Logo Logo

Citation: Galitz, Adrian and Vargas, Sergio and Thomas, Olivier P. and Reddy, Maggie M. and Wörheide, Gert and Erpenbeck, Dirk: Genomics of Terpene Biosynthesis in Dictyoceratid Sponges (Porifera) – What Do We (not) Know?. 28. July 2024. Open Data LMU. 10.5282/ubm/data.505

Genomics of Terpene Biosynthesis in Dictyoceratid Sponges (Porifera) – What Do We (not) Know?
Genomics of Terpene Biosynthesis in Dictyoceratid Sponges (Porifera) – What Do We (not) Know?

Sponges are recognized as promising sources for novel bioactive metabolites. Among them are terpenoid metabolites that constitute key biochemical defense mechanisms in several sponge taxa. Despite their significance, the genetic basis for terpenoid biosynthesis in sponges remains poorly understood. Dictyoceratida comprise demosponges well-known for their bioactive terpenoids. In this study, we checked the currently available genomic data for insights into the metabolic pathways of dictyoceratid terpenoids. We first identified prenyltransferase (PT) and terpene cyclase (TC) enzymes essential for the terpenoid biosynthetic processes in the terrestrial realm by analyzing available transcriptomic and genomic data of Dictyoceratida sponges and 10 other sponge species. All Dictyoceratida sponges displayed various PTs involved in either sesqui- or diterpene, steroid and carotenoid production. Additionally, it was possible to identify a potential candidate for a dictyoceratid sesterterpene PT. However, analogs of common terrestrial TCs were absent, suggesting the existence of a distinct or convergently evolved sponge-specific TC. Our study aims to contribute to the foundational understanding of terpene biosynthesis in sponges, unveiling the currently evident genetic components for terpenoid production in species not previously studied. Simultaneously, it aims to identify the known and unknown factors, as starting point for biochemical and genetic investigations in sponge terpenoid production.

Not available
Galitz, Adrian
Vargas, Sergio
Thomas, Olivier P.
Reddy, Maggie M.
Wörheide, Gert
Erpenbeck, Dirk
2024

[thumbnail of Protein Alignment] Other (Protein Alignment)
Alignment_Galitz_et_al_Genomics_Terpene Biosynth.nex - Supplemental Material

355kB
[thumbnail of README_Galitz_et_al.txt] Plain Text
README_Galitz_et_al.txt - Additional Metadata

498B

DOI: 10.5282/ubm/data.505

This dataset is available unter the terms of the following Creative Commons LicenseCC BY 4.0

Abstract

Sponges are recognized as promising sources for novel bioactive metabolites. Among them are terpenoid metabolites that constitute key biochemical defense mechanisms in several sponge taxa. Despite their significance, the genetic basis for terpenoid biosynthesis in sponges remains poorly understood. Dictyoceratida comprise demosponges well-known for their bioactive terpenoids. In this study, we checked the currently available genomic data for insights into the metabolic pathways of dictyoceratid terpenoids. We first identified prenyltransferase (PT) and terpene cyclase (TC) enzymes essential for the terpenoid biosynthetic processes in the terrestrial realm by analyzing available transcriptomic and genomic data of Dictyoceratida sponges and 10 other sponge species. All Dictyoceratida sponges displayed various PTs involved in either sesqui- or diterpene, steroid and carotenoid production. Additionally, it was possible to identify a potential candidate for a dictyoceratid sesterterpene PT. However, analogs of common terrestrial TCs were absent, suggesting the existence of a distinct or convergently evolved sponge-specific TC. Our study aims to contribute to the foundational understanding of terpene biosynthesis in sponges, unveiling the currently evident genetic components for terpenoid production in species not previously studied. Simultaneously, it aims to identify the known and unknown factors, as starting point for biochemical and genetic investigations in sponge terpenoid production.

Source

Chemistry and Biodiversity

Item Type:Data
Contact Person:Erpenbeck, Dirk
E-Mail of Contact:erpenbeck at LMU.de
Subjects:Biology
Dewey Decimal Classification:500 Natural sciences and mathematics > 570 Life sciences
500 Natural sciences and mathematics > 590 Zoological sciences
ID Code:505
Deposited By: PD Dr. Dirk Erpenbeck
Deposited On:23. Jul 2024 13:55
Last Modified:19. Aug 2024 07:40

Repository Staff Only: item control page