Data to: First report on presence of mitochondrial introns in freshwater sponges, and pseudogenic evidence of their loss

699 25 4235 5 16810 document 4235 Eunapius_RT_alignment.phy text/plain 4aa894d1cba0673aac8ce0cd9f531912 MD5 54963 2025-10-15 10:54:06 https://data.ub.uni-muenchen.de/699/1/Eunapius_RT_alignment.phy 699 1 1 text/plain text/plain Phylip alignment file en public

Eunapius_RT_alignment.phy

other 4336 3 17155 document 4336 Readme.txt text/plain 6e5bd4f0e6c2f504671b2d89b732f231 MD5 1957 2025-11-03 12:35:41 https://data.ub.uni-muenchen.de/699/4/Readme.txt 699 4 4 text/plain text/plain README de public

Readme.txt

other 4337 2 17159 document 4337 indexcodes.txt text/plain 08c81a77e169abec426e3d605de9a985 MD5 1013 2025-11-03 12:36:30 https://data.ub.uni-muenchen.de/699/5/indexcodes.txt 699 5 5 text/plain other Generate index codes conversion from text/plain to indexcodes de public

indexcodes.txt

http://eprints.org/relation/isVersionOf https://data.ub.uni-muenchen.de/id/document/4336 http://eprints.org/relation/isVolatileVersionOf https://data.ub.uni-muenchen.de/id/document/4336 http://eprints.org/relation/isIndexCodesVersionOf https://data.ub.uni-muenchen.de/id/document/4336 archive 158 disk0/00/00/06/99 2025-11-03 12:49:05 2026-01-13 14:49:27 2025-11-03 12:49:05 data show Mitochondrial introns have a patchy distribution in sponges lineages. Here, we report on the finding of a group-II-intron in Eunapius rarus (Demospongiae, Spongillidae), which constitutes the first report of a mitochondrial intron in freshwater sponges. Group-II-introns are self-splicing ribozymes, and are particularly rare among sponge mitochondrial genomes. The intron contains complete open reading frames (ORFs), including typical intron-encoded proteins (IEPs). Phylogenetic analysis reveals that the intron is more closely related to those found in brown algae, and distant from other sponge group-II-introns, indicating an acquisition of this intron independent from other sponges. Remarkably, the congeneric E. fragilis does not possess this intron in their mitochondrial genome. However we found pseudogenic copies of the E. rarus group-II-intron in the nuclear genome of E. fragilis, which indicates patterns of group-II-intron presence and their pseudogene transposition into the nuclear genomes in sponges for the first time. Our results show that a group-II-intron must have been present in the last common ancestor of both Eunapius mt genomes, and subsequently lost in E. fragilis, rather than independent acquisition. Consequently, our findings provide an explanation for the patchy distribution of introns in sponges as a result of frequent losses, besides multiple acquisitions. eng Zhao Zhen Ma Junye Yang Qun Wörheide Gert Erpenbeck Dirk 2025-10-15 500 550 590 10.5282/ubm/data.699 https://doi.org/10.5282/ubm/data.699 public group-II-intron mitochondrial intron Porifera freshwater sponges Numt Eunapius en cc-by Erpenbeck Dirk fak19 Data to: First report on presence of mitochondrial introns in freshwater sponges, and pseudogenic evidence of their loss