The aim of the research project was to test the validity and reliability of new methods of caries diagnostics on extracted human molars in vitro. A total of 375 teeth were examined. Microtomographic images were taken as a reference standard. Radiographs of these teeth and white light images from the occlusal, proximal, buccal and oral directions were taken as a comparative imaging technique to the new methods. As new examination methods, tranisillumination images were taken from the occlusal direction using near-infrared light with wavelengths of 1050, 1200 and 1300 nm with two different indium gallium arsenide (InGaAs) cameras. On the other hand, reflection images of the occlusal, buccal and oral areas were taken using near-infrared light with wavelengths of 1050, 1200, 1300 and 1550 nm, also with two InGaAs cameras. The cameras differed in the size of their sensor, with 320x256 and 640x512 pixels respectively. This is the near-infrared reflection image data set of the 375 teeth. Other data publications from the "In-vitro caries diagnostic study": https://doi.org/10.5282/ubm/data.441 (near-infrared transillumination images at 1050, 1200 and 1300 nm from occlusal of 375 extracted human teeth) https://doi.org/10.5282/ubm/data.444 (375 bitewing radiographs of extracted human teeth) https://doi.org/10.5282/ubm/data.445 (375 microtomographic images of the coronal part of extracted human teeth) https://doi.org/10.5282/ubm/data.446 (white light images from occlusal, proximal, buccal and oral direction of 375 extracted human teeth)