Lateral root primordia development of wild type and pip mutants of Arabidopsis thaliana

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Col.avi

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pip2_1_sample1.avi

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pip2_1_sample2.avi

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pip2_8_sample1.avi

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pip2_8_sample2.avi

supplemental archive 912 disk0/00/00/03/41 2022-11-25 09:56:14 2022-11-25 09:56:24 2022-11-25 09:56:14 data show The experiment was performed on Arabidopsis thaliana wild type (Col) and aquaporin single mutants, pip2;1-2 and pip2;8-2, labeled with a marker for plasma membrane (UBQ10pro::PIP1;4-3xGFP) and nuclei of LRP cells (GATA23pro::H2B:3xmCHERRY) in a culture medium with ½ Murashige-Skoog and 0.8% Phytoagar. The plants were grown vertically for four days, and the gravitropic stimulus (pgi) was applied by rotating the plates at 180 °. At 17 hours pgi, the seedlings were transferred to a light-sheet microscope, and the LRP development was recorded every 30 min from 18 h to 39 h pgi. Images were processed, drift corrected, and cropped using the BigDataProcessor (Tischer et al. 2019) in Fiji (Schindelin et al. 2012). Max Projection was applied after imaging processing. eng Schley Thayssa 2022-10-10 570 580 10.5282/ubm/data.341 https://doi.org/10.5282/ubm/data.341 public en cc-by-sa fak18 fak19 Lateral root primordia development of wild type and pip mutants of Arabidopsis thaliana