Raw data points and images from the article "Prebiotic Gas Flow Environment Enables Isothermal Nucleic Acid Replication"
by Dr. Philipp Josef Schwintek, Emre Eren, Dr. Christof Mast, and Prof. Dieter Braun*. Preprint is available at
https://elifesciences.org/reviewed-preprints/100152.

This work was supported by the Deutsche Forschungsgemein-324
schaft (DFG, German Research Foundation) – Project-ID 364653263 – CRC 235, Deutsche Forschungs-325
gemeinschaft (DFG, German Research Foundation) – Project-ID 521256690 – CRC 392, Deutsche326
Forschungsgemeinschaft (DFG, German Research Foundation) – Project-ID 201269156 – SFB 1032,327
Volkswagen Initiative’ Life? – A Fresh Scientific Approach to the Basic Principles of Life’, HFSP328
RGP003/2023, Germany’s Excellence Strategy EXC-2094-390783311, Simons Foundation #327125,329
European Research Council EvoTrap #787356, ERC-2017-ADG. This work was also supported by the 330
Center for Nanoscience Munich (CeNS).

Contact person for the project:

Prof. Dieter Braun,
Fakultaet fuer Physik,
Ludwig-Maximilians-Universitaet Muenchen
Geschwister-Scholl-Platz 1
80539 Muenchen

The data stored in the research data repository of the University Library of Munich (Open Data LMU) as a zip file 
is composed as follows:

1. 

Folder "Figure 2": Contains "Raw Data Points" folder in which the raw data of the graphs displayed in figure 
2 is stored either in .txt or .ods files and the "Raw Images" Folder in which raw .tif files captured by the microscope are 
stored (For beads). For the DNA Accumulation, the files are in .jpg format, as their values correspond to c/c0 of dissolved DNA.

2.

Folder "Figure 3": Contains "Raw Data" folder in which the raw data of the graphs displayed in figure 
3 is stored in .txt files and the "Figure  3a FRET Images" Folder in which .jpg files captured by the microscope are 
stored (For FRET Values). It also contains the "Raw Images" folder in which the raw .tif files, captured by the FRET
microscope are stored. Additionally, the raw .tif images from the melting curve used to determine the alpha and beta values
are stored in the folder "Melting Curve"

3. 

Folder "Figure 4": Contains "Raw Data Points" folder in which the raw data of the graphs displayed in figure 
4c is stored either in a .txt file. Contains "Raw Images" Folder in which the raw images of all 3 repeats of FLUX ON and FLUX OFF measurements
are stored as .tif files.

4. 

Folder "Figure 3-figure supplement 2" containts the data used in form of .pxp files (Igor Pro file format, can however be opened 
with other free software as well. Additionally, .PDF files containing the graphs are stored here.

5.

Folder "Figure 3-figure supplement 3" containts the data used in form of .pxp files (Igor Pro file format, can however be opened 
with other free software as well. Additionally, .PDF files containing the graphs are stored here.

6.

Folder "Appendix 9-figure 1" containts the data used in form of .pxp files (Igor Pro file format, can however be opened 
with other free software as well. Additionally the data is saved in a tab delimited .txt file.

7.

The file "COMSOL Simulation File Schwintek et al.mph" is the COMSOL Multiphysics 5.4 simulation file used throughout the paper.
It contains DNA accumulation simulations, evaporation dynamics, flowspeeds, MgCl accumulation as well as calculated results and images.

8.

The folder LabView Programs contains two folders. One containing the FRET analysis tool used to calculate the FRET per pixel values
for figure 3 using the images stored in "Figure 3\Raw Images". The other containing the Program used to calculate the salt and product concentration along the random walk path
stored in "Figure 3\Raw Data".