README This is the alignment used for the work: "Bangkok urban census of aquatic invertebrate fauna by an integrative taxonomic approach: the case of Suan Luang Rama IX Park and its freshwater sponges (Spongillida: Spongillidae)" Authored by Nisit Ruengsawan, Narumon Sangpradub. Tiziana Cubeddu, Dirk Erpenbeck, Gert Wörheide, Roberto Pronzato & Renata Manconi. This nucleotide alignment of the Internal Transcribed Spacer region is in nexus format and used for phylogenetic reconstructions, and consists of 8 taxa with an alignment length of 748 characters.